Massively Parallel Sequencing of Forensically Relevant Single Nucleotide Polymorphisms Using TruSeq (TM) forensic amplicon

• NCJ Number: 249352
• Journal: International Journal of Legal Medicine Volume: 129 Issue: 1 Dated: January 2015 Pages: 31-36
• Author(s): David H. Warshauer; Carey P. Davis; Cydne Holt; Yonmee Han; Paulina Walichiewicz; Tom Richardson; Kathryn Stephens; Anne Jager; Jonathan L. King; Bruce Budowle
• Date Published: January 2015
• Length: 6 pages
• Grant Number(s): 2012-DN-BX-K033
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical); Report (Technical); Report (Study/Research); Report (Grant Sponsored); Program/Project Description
• Award: Development of Reference Sample DNA Profiling for Databases Using Next Generation Sequencing Technologies

Annotation

This study used the TruSeq Forensic Amplicon library preparation protocol to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples.

Abstract

The TruSeq Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. Results were com- pared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS). (Publisher abstract modified)