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Massively Parallel Sequencing of Forensically Relevant Single Nucleotide Polymorphisms Using TruSeq (TM) forensic amplicon

NCJ Number
Date Published
January 2015
6 pages
This study used the TruSeq™ Forensic Amplicon library preparation protocol to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples.
The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. Results were com- pared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS). (Publisher abstract modified)
Date Published: January 1, 2015