In this procedure, the RP reacts with europium chloride hexahydrate to form the organo-rare earth complex which exhibits europium luminescence at 615 nanometer with a lifetime of 0.4 milliseconds. Dye laser excitation together with absorption spectral data suggest that the fingerprint luminescence arises not only from direct excitation of the europium ion in the complex, but mainly by resonance energy transferred from the ligand to the europium ion of the complex. Experimentation with benzo(f)ninhydrin showed the luminescence of the europium complex as well as the europium emission under near-ultraviolet excitation to be more intense than that of a comparable ninhydrin complex. Because of its long-lived luminescence, the europium complex is thus suited for time-resolved imaging for eliminating short-lived or background fluorescence on porous surfaces to reveal amino acids images. 6 figures and 17 references (Author abstract modified)
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