NCJ Number
212662
Journal
Journal of Forensic Sciences Volume: 50 Issue: 6 Dated: November 2005 Pages: 1481-1485
Date Published
November 2005
Length
5 pages
Annotation
Toxicological evaluation of postmortem urine collected from a 41-year-old deceased White male detected anhydroecgonine ethyl ester (Ethylecgonidine, AEEE), a transesterification product of smoked cocaine abused in conjunction with ethanol.
Abstract
Descriptions of the materials and methods used in the procedure address synthetic procedures, instrumentation, the extraction procedure, and standard curve generation. A solid phase extraction (SPE) method was used to extract cocaine, AEEE, and related metabolites from the deceased's urine. SPE on a 1 ml urine sample from the decedent, followed by gas chromatography-mass spectrometry (GC-MS) detected AEEE. Other metabolites identified by GC-MS included cocaine, cocaethylene, and anhydroecgonine methyl ester (AEME). To determine whether some or all of the AEEE was artifactually produced in the heated GC injector port, an alternative liquid chromatography-mass spectrometry (LC-MS) method was developed. LC/MS following SPE found at least 50 ng/ml of AEEE in the extract. The mass fragmentation of AEEE detected in the urine was compared to spectra of authentic, synthesized compound. AEEE is thus a potential additional forensic marker for the co-abuse of smoked cocaine and ethanol. LC-MS provides the basis for a useful analytical method in forensic cases involving suspected free-base cocaine/ethanol use. 36 references
Date Published: November 1, 2005
Downloads
No download available
Similar Publications
- Correctional Officer Fatalities in Line of Duty During 2005 to 2015: A Survival Analysis
- Using Technology to Enhance Police Problem Solving, Final Report
- Role of Drug and Alcohol Abuse in Domestic Violence and Its Treatment: Dade County's Domestic Violence Court Experiment, Appendices to the Final Report