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Genetic Markers in Human Bone: I. Deoxyribonucleic Acid (DNA) Analysis

NCJ Number
128900
Author(s)
D T Anderson, K M Berka, N L Folk, R E Gaensslen, D D Garner, G L Herrin, T P Keith, H C Lee, E M Pagliaro, P Phipps, G Ruano
Date Published
January 1991
Length
11 pages
Annotation
Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a "per milligram of starting tissue" basis.
Abstract
DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different single-locus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQa phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3' to the human apolipoprotein (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization. 5 figures and 49 references (Author abstract)

Date Published: January 1, 1991