U.S. flag

An official website of the United States government, Department of Justice.

Forensic Molecular Biomarkers for Mixture Analysis

NCJ Number
Fabio Oldoni; Daniele Podini
Date Published
July 2019
13 pages
This review describes novel, forensically relevant multi-SNP loci (i.e., microhaplotypes or microhaps), compound markers (i.e., DIP-STRs, SNP-STRs and DIP-SNPs) and lineage markers (i.e., rapidly mutating Y chromosome STRs) that improve the deconvolution of two and more than two-person mixtures typed using conventional STR, binary and non-binary loci.
The deconvolution of DNA mixtures has gained the attention of forensic DNA scientists for over two decades. To enhance mixture deconvolution capabilities, a new generation of sensitive DNA-typing approaches has been recently prod. The current review explores the features and applications of these emerging molecular biomarkers with respect to their ability to forensically detect same-or-opposite sex donors. It also discusses the impact of initial massively parallel sequencing (MPS) investigations of STR, microhaplotype, and SNP/indel assays for DNA mixture profiling. (publisher abstract modified)

Date Published: July 1, 2019