This article examines whether a linear or exponential model best describes the relationship between peak height and molecular weight, with the aim of improving the ability to model peak heights for use in DNA interpretation software.
Selected profiles typed at the Promega PowerPlex 21 (PP21) loci were examined to determine if a linear or exponential model best described the relationship between peak height and molecular weight. There were fewer large departures from observed and expected peak heights using the exponential model. The larger differences that were observed were exclusively at the high molecular weight loci. The authors conclude that the data supports the use of an exponential curve to model peak heights versus molecular weight in PP21 profiles. The authors believe this observation will improve the ability to model expected peak heights for use in DNA interpretation software. (Publisher Abstract Provided)