Concordance Study Between Miniplex Assays and a Commercial STR Typing Kit

• NCJ Number: 241198
• Author(s): Jiri Drabek Ph.D.; Denise T. Chung B.S.; John M. Butler Ph.D.; Bruce R. McCord Ph.D.
• Date Published: July 2004
• Length: 2 pages
• Grant Number(s): 2002-IJ-CX-K007
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical)
• Award: Novel STR Multiplexes with Reduced Size To Analyze DNA

Annotation

This study investigated the concordance between Miniplex assays and a commercial STR typing kit.

Abstract

This study investigated the concordance between Miniplex assays and a commercial STR typing kit and found that of the 532 samples evaluated, full concordance was observed in 99.77 percent of STR allele calls compared (6,369 STR alleles out of a total of 6,384 STR alleles). For those alleles that were not in concordance, primer binding site mutations were identified and alterations were made to the Miniplex system to correct for discrepancies in future studies. Data for the study were obtained from analysis of 532 anonymous liquid blood samples. The samples were extracted, quantified, and typed using the Applied Biosystems AmpFLSTR Identifiler kit. In addition, Miniplex short tandem repeat (STR) assays were used and run on an ABI Prism 310 Genetic Analyzer. For each of the samples, a total of 12 STR loci were compared between the single amplification Identifiler it and the 3 separate Miniplex sets. The analysis found almost complete concordance between the two identification methods. Plans for future research are discussed. Table and references