Since cleaning the surface of bone samples is a necessary step to remove contaminants prior to isolating DNA for forensic DNA analysis, the current project developed a simple trypsin method for cleaning bone samples prior to DNA isolation.
Cleaning the surface of human bone samples was achieved by the application of trypsin solution. Light microscopy and scanning electron microscopy results indicated that trypsin treatment was effective in removing the outer surface of bone samples. The yield of DNA isolated from trypsin-treated bone samples was sufficient for subsequent short tandem repeat (STR) analysis. STR analysis revealed no adverse effect on the DNA profile after the trypsin treatment. The data suggest that this trypsin method can potentially be an alternative cleaning method to mechanical cleaning methods. (publisher abstract modified)
Downloads
Related Topics
DNA (Deoxyribonucleic Acid)Similar Publications
- The Study of Tissue-Specific DNA Methylation as a Method for the Epigenetic Discrimination of Forensic Samples
- Large-scale Selection of Highly Informative Microhaplotypes for Ancestry Inference and Population Specific Informativeness
- Targeted Enrichment of Whole-genome SNPs from Highly Burned Skeletal Remains