A total of 260 U.S. Caucasians, 260 African-Americans, and 140 U.S. Hispanics were typed for 27 Y-chromosome short tandem repeat (Y-STR) markers; for six of the loci, only a subset of each population group was examined that included 31 Caucasians, 32 African-Americans, and 32 Hispanics.
The 27 STR are DYS444, DYS446, DYS449, DYS463, DYS485, DYS490, DYS495, DYS504, DYS505, DYS508, DYS520, DYS522, DYS525, DYS532, DYS533, DYS534, DYS540, DYS556, DYS557, DYS570, DYS575, DYS576, DYS594, DYS632, DYS635, DYS641, and DYS643. The data presented are preliminary; however, apparently several simple repeat loci, including DYS570 and DYS576 are highly polymorphic; thus, they may aid resolution of common Y-STR haplotypes that cannot be separated with the widely used minimal haplotype loci. One table presented compares the gene diversity values for the individual loci in all the samples examined, as well as the various U.S. population groups. Blood samples were extracted using a modified salting out procedure. Extracted DNA was quantified using UV spectrophotometry followed by a PicoGreen assay in order to adjust concentrations to approximately 1 ng/mL. 3 tables, 1 figure, and 13 references
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