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Resolution of DNA Mixtures and Analysis of Degraded DNA Using the 454 DNA Sequencing Technology

Award Information

Award #
2010-DN-BX-K141
Funding Category
Competitive
Location
Awardee County
Alameda
Congressional District
Status
Closed
Funding First Awarded
2010
Total funding (to date)
$699,230

Description of original award (Fiscal Year 2010, $699,230)

The researchers propose to utilize the next-generation sequencing platform, 454 Roche DNA sequencing technology to establish a method to analyze mixed, limited, and/or degraded DNA samples using mtDNA and miniplex STR markers simultaneously. This project will result in two deliverables: (1) a validated "front-end" system targeting both nuclear STR and mtDNA markers for use with the 454 sequencing technology for application to forensically relevant samples (i.e. degraded and mixed); and (2) Software for the interpretation of data generated by the system.

ca/ncf

Date Created: August 31, 2010