Identification and Separation of Evidence Mixtures Using SNP-Based FISH Techniques and Laser Microdissection

Previous research has demonstrated the capability to successfully separate male/female cellular mixtures using Fluorescent In Situ Hybridization (FISH) probes combined with lasermicrodissection microscopy. The goal of the current project is to expand the previous work and employ single nucleotide polymorphism (SNPs) in a FISH-based assay to visually detect and physically separate the components of a mixture of cells from individuals of the same gender. The separated cells will then be made available for subsequent DNA analysis. This work will advance the ability to successfully perform DNA analysis on biological evidence containing DNA from multiple contributors.

ca/ncf