This awardee has received supplemental funding. This award detail page includes information about both the original award and supplemental awards.
Description of original award (Fiscal Year 2003, $202,513)
Project Summary 2003-ij-cx-k104
This award aims to demonstrate the reliability of DHPLC to detect and clearly resolve mixtures of mitochondrial DNA or all classes of mtDNA polymorphisms including c-stretch variants, insertion/deletion variants and sequence polymorphisms in both GC -rich and AT- rich areas in the HVI and HVII region of the human mtDNA 2) to efficiently seperate and recover individual components of DNA mixture for subsequent analyisis by direct DNA sequencing and then to confirm the accuracy of the resulting sequence data and 3) with regards to the absence of cross-contamination between individual samples in the sample tray and between individual assays.
The first phase of this project examined the feasibility of DHPLC to successfully separate mtDNA mixtures that differed at one or more positions in the mtDNA hypervariable regions (HV1 and HV2). The grantee has demonstrated that DHPLC can successfully be used for this purpose. This supplemental project will develop Standard Operating Procedures and interpretation guidelines for mtDNA mixture analysis using DHPLC-separated fractions, and perform developmental validation studies in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM).
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