Mitochondrial DNA Analysis by Denaturing Liquid Chromatography for the Separation of Mixtures in Forensic Samples

The first phase of this project examined the feasibility of DHPLC to successfully separate mtDNA mixtures that differed at one or more positions in the mtDNA hypervariable regions (HV1 and HV2). The grantee has demonstrated that DHPLC can successfully be used for this purpose. This supplemental project will develop Standard Operating Procedures and interpretation guidelines for mtDNA mixture analysis using DHPLC-separated fractions, and perform developmental validation studies in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM).

nca/ncf