0:00 okay there's the formula you use and RC
0:03 - which is that yellow book
0:05 recommendation 4.1 they ask you to do a
0:08 correction on the homozygotes right
0:11 instead of just using P squared which is
0:13 hardy-weinberg they're telling you to
0:16 say P squared and then add that thing to
0:18 the right of it which is just P which is
0:22 the allele frequency for that locus
0:26 you're looking at times 1 minus the
0:29 allele frequency times 0.01 right so try
0:32 it say your allele frequency is 0.3
0:37 what's 0.3 squared is what wait oh 9
0:44 plus 0.3 times 0.7 is what point to 1
0:51 what's 0.2 1 times 0.01
0:56 just shift the decimal place and add
1:00 that it's a point o night when you get a
1:05 oh nine right
1:12 is that much different than point on
1:14 mine
1:17 so with common alleles it doesn't change
1:23 it much right so that if you have common
1:29 alleles and you add this correction
1:32 factor for population stratification or
1:35 population inbreeding which could lead
1:38 to a departure and hardy-weinberg
1:39 equilibrium if it existed for common
1:43 alleles it's not going to change it much
1:44 at all right why don't we correct
1:49 heterozygotes when you have inbreeding
1:53 you're increasing homozygosity okay so
1:57 what they're trying to do is we're
1:58 saying well if there is in breeding you
2:01 want to correct so that making the
2:06 homozygotes more common right to account
2:12 for possible in reading so what they're
2:14 saying is your population numbers that
2:18 you're calculating aren't accounting for
2:21 in breeding so they're too rare so you
2:26 got to make your homozygotes more common
2:28 to be more reflective if there was in
2:31 breeding you see that you're you're
2:35 increasing the frequency of the
2:38 homozygous as if there was one percent
2:41 in breeding your heterozygotes if there
2:47 was are going to be an overstatement of
2:49 what they should be
