Using DNA previously extracted from 200 unrelated individuals from Minnesota, this study compared Y-STR concordance among seven kits.
The kits tested are the Y-PlexTM 5, Y-PlexTM 6, Y-PlexTM 12, Powerplex Y, Y-FilerTM, Multiplex I (MPI), and MPII. Amplification of between 0.5 and 1.0 ng of DNA was performed. The study demonstrates, except for the discrepancies noted, that all primer sets currently available will produce concordant results. Significantly, there was a 100 percent concordance between the typing results obtained from the two most commonly used commercial Y-STR systems, i.e., Powerplex Y and YfilerTM kits. This indicates that results obtained for forensic samples can be compared even when using different amplification kits. In addition, databases constructed using one amplification kit may be used to determine the frequency of a particular haplotype obtained using a different amplification kit. Three discrepancies are explained, accompanied by explanations as to why they do not discount the main conclusion of the study. 2 tables and 11 references
Downloads
Similar Publications
- Mitochondrial DNA Analysis by Denaturing High-Performance Liquid Chromatography for the Characterization and Separation of Mixtures in Forensic Samples
- The Collection, Preservation, and Processing of DNA Samples from Decomposing Human Remains for More Direct Disaster Victim Identification (DVI)
- Discrimination Between Human and Animal Blood Using Raman Spectroscopy and a Self-Reference Algorithm for Forensic Purposes: Method Expansion and Validation