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Validating Multiplexes for Use in Conjunction With Modern Interpretation Strategies

NCJ Number
Forensic Science International-Genetics Volume: 20 Dated: January 2016 Pages: 6-19
Date Published
January 2016
14 pages
This article presents a full scheme for validation of a new multiplex that is suitable for informing modern interpretation practice, predominantly using GlobalFiler as an example multiplex; however, it is suggested that the aspects investigated here are fundamental to introducing any multiplex in the modern interpretation environment.

In response to requests from the forensic community, commercial companies are generating larger, more sensitive, and more discriminating STR multiplexes. These multiplexes are now applied to a wider range of samples including complex multi-person mixtures. In parallel there is an overdue reappraisal of profile interpretation methodology. Aspects of this reappraisal include 1) the need for a quantitative understanding of allele and stutter peak heights and their variability; 2) an interest in reassessing the utility of smaller peaks below the often used analytical threshold; 3) a need to understand not just the occurrence of peak drop-in but also the height distribution of such peaks; and 4) a need to understand the limitations of the multiplex-interpretation strategy pair implemented. (Publisher abstract modified)

Date Published: January 1, 2016