Validating Multiplexes for Use in Conjunction With Modern Interpretation Strategies

• NCJ Number: 250072
• Journal: Forensic Science International-Genetics Volume: 20 Dated: January 2016 Pages: 6-19
• Author(s): D. Taylor; J. A. Bright
• Date Published: January 2016
• Length: 14 pages
• Grant Number(s): 2011-DN-BX-K541
• Agencies: NIJ-Sponsored
• Award: Population Genetic Issues for Forensic DNA Profiles

Annotation

This article presents a full scheme for validation of a new multiplex that is suitable for informing modern interpretation practice, predominantly using GlobalFiler as an example multiplex; however, it is suggested that the aspects investigated here are fundamental to introducing any multiplex in the modern interpretation environment.

Abstract

In response to requests from the forensic community, commercial companies are generating larger, more sensitive, and more discriminating STR multiplexes. These multiplexes are now applied to a wider range of samples including complex multi-person mixtures. In parallel there is an overdue reappraisal of profile interpretation methodology. Aspects of this reappraisal include 1) the need for a quantitative understanding of allele and stutter peak heights and their variability; 2) an interest in reassessing the utility of smaller peaks below the often used analytical threshold; 3) a need to understand not just the occurrence of peak drop-in but also the height distribution of such peaks; and 4) a need to understand the limitations of the multiplex-interpretation strategy pair implemented. (Publisher abstract modified)