Depth of coverage variation and strand bias were identified but generally were infrequent and did not impact reliability of variant calls. Multiplexing of samples was demonstrated, which can improve throughput and reduce cost per sample analyzed. Overall, the results of this study, based on orthogonal concordance testing and phylogenetic scrutiny, supported that whole mtGenome sequence data with high accuracy can be obtained using the PGM platform. Depth of coverage variation and strand bias were identified but generally were infrequent and did not impact reliability of variant calls. Multiplexing of samples was demonstrated which can improve throughput and reduce cost per sample analyzed. Overall, the results of this study, based on orthogonal concordance testing and phylogenetic scrutiny, supported that whole mtGenome sequence data with high accuracy can be obtained using the PGM platform. (Publisher abstract modified)
Downloads
Similar Publications
- The Role of Simulated Data in Making the Best Predictions (from the 87th Annual Meeting of the American Association of Physical Anthropologists - 2018)
- QCM-based screening of acrylate polymers for NPPA pre-concentration to enhance vapor detection of fentanyl
- Technical Note: A novel method for simultaneous recovery of DNA, RNA, and proteins from trace biological samples for forensic application