SERS-based plasmonic nanobiosensing in single living cells

• NCJ Number: 304485
• Journal: Analytical and Bioanalytical Chemistry Volume: 393 Dated: 2009 Pages: 1135–1141
• Author(s): Jonathan P. Scaffidi; Molly K. Gregas; Victoria Seewaldt; Tuan Vo-Dinh
• Date Published: 2009
• Length: 7 pages
• Grant Number(s): 2006-DN-BX-K034
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical)
• Award: Development of Advanced Raman Spectroscopy Methods and Databases for the Evaluation of Trace Evidence and the Examination of Questioned Documents

Annotation

This paper describes the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection.

Abstract

The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized “normal” human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level. (Publisher Abstract)