DNA re-identification is used for a broad suite of applications, ranging from cell line authentication to forensics; however, current re-identification schemes suffer from high latency and limited access. In addressing this issue, MinION sketching requires as few as 3 minutes of sequencing and 60-300 random SNPs to re-identify a sample, enabling near real-time applications of DNA reidentification. The method used in the current project capitalizes on the rapidly growing availability of genomic reference data for cell lines, tissues in biobanks, and individuals. This empowers the application of MinION sketching in research and clinical settings for periodic cell line and tissue authentication. Importantly, this method enables considerably faster and more robust cell line authentication relative to current practices and could help to minimize the amount of irreproducible research caused by mix-ups and contamination in human cell and tissue cultures. (publisher abstract modified)
Downloads
Similar Publications
- Enhanced Sensitivity and Homogeneity of SERS Signals on Plasmonic Substrate When Coupled to Paper Spray Ionization-Mass Spectrometry
- Design of Light-Induced Solid-State Plasmonic Rulers via Tethering Photoswitchable Molecular Machines to Gold Nanostructures Displaying Angstrom Length Resolution
- Mitochondrial DNA Control Region Variation in a Kuwaiti Population Sample