Quadriplex real-time PCR (qPCR) assay for human-canine-feline species identification and nuclear DNA quantification

• NCJ Number: 255424
• Journal: Forensic Science International: Genetics Volume: 6 Dated: May 2012 Pages: e97-e98
• Author(s): Sreetharan Kanthaswamy; A. Premasuthan
• Date Published: May 2012
• Length: 0 pages
• Grant Number(s): 2008-DN-BX-K288
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical)
• Award: Development of a DNA-Based Multi-Species Identification and Quantification Assay

Annotation

This letter is an update to a recent publication in the Forensic Science International Genetics journal, “Quantitative real-time PCR (qPCR) assay for human–dog–cat species identification and nuclear DNA quantification”: S. Kanthaswamy, A. Premasuthan, et al.

Abstract

This triplex qPCR assay quantifies and identifies the presence of as little as 0.4 pg of human, feline and canine DNA in a mixed sample [ [1] ]. The assay did not include an Internal Positive Control (IPC) due to the three-dye limit in the Applied Biosystems 7300 machine. The assay is now modified to include an IPC using the 7500 Fast Real Time PCR system. 1 figure and 3 references (publisher abstract modified)