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An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained From Archived Latent Fingerprints

NCJ Number
Date Published
April D. Solomon M.S., Madison E. Hytinen B.S., Aryn M. McClain M.S., Marilyn T. Miller Ed.D., Tracey Dawson Cruz Ph.D.
Although DNA profiles have been obtained from fingerprints, there is limited knowledge regarding DNA analysis from archived latent fingerprints (touch DNA “sandwiched” between adhesive and paper), so the current study compared a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type.
Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri Sep™ columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100 percent of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases. (publisher abstract modified)
Date Created: April 3, 2019