A novel and in situ technique for the quantitative detection of MTBE and benzene degrading bacteria in contaminated matrices

• NCJ Number: 311676
• Journal: Journal of Microbiological Methods Volume: 68 Issue: 2 Dated: February 2007 Pages: 437-441
• Author(s): J.P. Biggerstaff; M. Le Puil; B.L. Weidow; J. Leblanc-Gridley; E. Jennings; J. Busch-Harris; K.L. Sublette; D.C. White; Randall S. Alberte
• Date Published: February 2007
• Length: 5 pages
• DOI: 10.1016/j.mimet.2006.09.016
• Grant Number(s): 2004-LP-CX-K048
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical)
• Award: Streamlining Physical Evidence Collection and Forensic Analysis.

Abstract

A novel and in situ technique is presented here as a better alternative to culture-dependent and PCR-based techniques for the quantitative detection of predominant bacterial species involved in the bioremediation of contaminants. It allowed rapid, specific and in situ identification of Biosep®-immobilized eubacteria from MTBE- and benzene-contaminated matrices.

(Publisher abstract provided.)