This article describes a qPCR assay that demonstrates it is possible to estimate the age of bloodstains with reasonable accuracy.
The value of RNA analysis in the forensic laboratory as one means of identifying the nature of biological evidence of forensic relevance has been well established. The degradation of RNA in dried body fluid stains has also been an area of forensic interest because of the potential to estimate the age of a stain recovered from a crime scene. The 5′–3′ qPCR assay advocated in the current article exploits the observation that the 5′ end of an mRNA transcript degrades in dried stains faster than the 3′ end. This differential degradation pattern can be followed with a qPCR assay that quantifies ∼90 bp amplicons produced from the 5′ and 3′ ends of 4 transcripts chosen from the transcriptome of blood because of their degradation kinetics, determined initially using RNA sequencing. Statistical analysis of degradation kinetics suggests, depending upon the age of the sample, the age of blood stains can be accurately estimated to within 2–4 weeks for stains less than 6 months of age and 4–6 weeks for stains 6 months to 1 year old. (publisher abstract modified)
Downloads
Similar Publications
- The Role of Simulated Data in Making the Best Predictions (from the 87th Annual Meeting of the American Association of Physical Anthropologists - 2018)
- Proteomic Genotyping for Individual Human Identification: Inferring SNPs in the Absence of DNA Evidence
- Recommended Scope for NPS Testing in the United States