The purpose of this technology transfer workshop is to provide the student with an introduction to laser microdissection (LMD), methods for preparing samples for LMD, LMD operation, methods for DNA analysis of LMD recovered cells, and an understanding of the application of LMD recovery to sexual assault evidence analysis. This class will include both lecture and laboratory activities to illustrate the concepts presented. Laser microdissection will be demonstrated on sperm and epithelial cells smears using the latest LMD instrumentation. Cells collected from the laboratory exercises will be analyzed for Short Tandem Repeat loci using PCR and capillary electrophoresis analysis.
Downloads
Similar Publications
- Role of Automation in the Examination of Handwritten Items (2012)
- Assessing the value of bacteria, plants, fungi and arthropods characterized via DNA metabarcoding for separation of forensic-like surface soils at varied spatial scales
- Examining longitudinal associations between polysubstance use and firearm-related risk behaviors from adolescence into emerging adulthood: a group-based multi-trajectory modeling approach