NCJ Number
236447
Date Published
July 2007
Length
5 pages
Annotation
The purpose of this technology transfer workshop is to provide the student with an introduction to laser microdissection (LMD), methods for preparing samples for LMD, LMD operation, methods for DNA analysis of LMD recovered cells, and an understanding of the application of LMD recovery to sexual assault evidence analysis.
Abstract
The purpose of this technology transfer workshop is to provide the student with an introduction to laser microdissection (LMD), methods for preparing samples for LMD, LMD operation, methods for DNA analysis of LMD recovered cells, and an understanding of the application of LMD recovery to sexual assault evidence analysis. This class will include both lecture and laboratory activities to illustrate the concepts presented. Laser microdissection will be demonstrated on sperm and epithelial cells smears using the latest LMD instrumentation. Cells collected from the laboratory exercises will be analyzed for Short Tandem Repeat loci using PCR and capillary electrophoresis analysis.
Date Published: July 1, 2007
Downloads
Similar Publications
- Questioning the prevalence and reliability of human mitochondrial DNA heteroplasmy from massively parallel sequencing data
- Assessing the value of bacteria, plants, fungi and arthropods characterized via DNA metabarcoding for separation of forensic-like surface soils at varied spatial scales
- Using posterior probability informed thresholds to develop best practice recommendations for MorphoPASSE using the innominate, cranial, and combined traits