Molecular analysis of the RNA transcriptome from a putative tissue fragment should permit the assignment of its source to a specific organ, since each will exhibit a unique pattern of gene expression. Determination of the organ source of tissues from crime scenes may aid in shootings and other investigations. The organs and tissues identified with the tested prototype profiling assay included brain, lung, liver, heart, kidney, intestine, stomach, skeletal muscle, adipose, and trachea. The biomarkers were chosen after iterative specificity testing of numerous candidate genes in various tissue types. The assay is very specific, with little cross-reactivity with non-targeted tissue, and can detect RNA mixtures from different tissues. Researchers also demonstrated the ability of the assay to identify the tissue source of origin using a single blind study. (publisher abstract modified)
Downloads
Similar Publications
- Detecting fentanyl analogs in counterfeit pharmaceuticals by surface-enhanced Raman spectroscopy using handheld Raman spectrometers
- Comprehensive Assessment of Novel Reference Standard Materials and Analytical Methods for the Analysis and Interpretation of Organic and Inorganic Gunshot Resid
- A Reflective Spectroscopy and Mineralogical Investigation of Cosmetic Blush (Wet‘N’Wild) Potentially for Forensic Investigations Related to Interpersonal Violence—An Experimental Feasibility Study