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Genome Sequence of a Providencia stuartii Strain Isolated From Lucilia sericata Salivary Glands

NCJ Number
Microbiology Resource Announcements Volume: 5 Issue: 17 Dated: 2017 Pages: 1-3
Date Published
3 pages

This article presents the draft genome sequence of a Providencia stuartii strain, derived from the salivary glands of larval Lucilia sericata, a common blow fly important to forensic, medical, and veterinary science.


The genome sequence will help dissect coinfections involving P. stuartii and Proteus mirabilis, as well as blow fly-bacteria interactions. The Genome Announcement states that Providencia stuartii is a Gram-negative bacillus bacterium that frequently causes urinary tract infections in hospital patients and has intrinsic resistance to antibiotics. Infections have been reported to progress to bacteremia, diarrhea, peritonitis, meningitis, infective endocarditis, and often co-occur with Proteus infections. In addition, Providencia species were reported to associate with blow flies, stable flies, Mexican fruit flies, vinegar flies, and house flies. Providencia can cause variable infections and different levels of mortality in their hosts and have been shown to impact blow fly attraction to resources. This article presents a draft genome of P. stuartii strain Crippen. The genomic sequence was isolated from a colony derived from maggot salivary glands of L. sericata third instars raised on beef liver. DNA sequencing was performed using an Ion Torrent personal genome machine (Life Technologies, Inc., Carlsbad, CA, USA) after preparation with a NEBNext fast DNA fragmentation library prep set. The sequencing data comprise 1,383,989 reads, with an average length of 212 bp, totaling 294 Mb. Sequence assembly using the PATRIC full assembly service produced 243 contigs, with an N50 of 191,375 bp and a total of 4.72 million nucleotides, resulting in approximately 62-fold coverage. This strain is comparably similar to three previously sequenced P. stuartii strains: MRSN 2154 (GenBank accession no. CP003488.1), ATCC 33672 (GenBank accession no. CP008920.1), and FDAARGOS_145 (GenBank accession no. CP014024.1). Further genome assemblies based on CONTIGuator indicate that the longest 37 contigs were mapped to the reference strain, with 93.8 percent of the assembled nucleotides aligned to the P. stuartii MRSN 2154 genome. No evidence of plasmids was found based on analysis with the PlasmidFinder version 1.3 server. This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number LVIE00000000 . The version described in this paper is the first version, LVIE01000000. 29 references (publisher abstract modified)

Date Published: January 1, 2017