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Fluorescently Labeled Bacteria Provide Insight on Post-mortem Microbial Transmigration

NCJ Number
251260
Date Published
Author(s)
Z. M. Burcham, J. A. Hood, J. L. Pechal, K. L. Krausz, J. L. Bose, C. J. Schmidt, M. E. Benbow, H. R. Jordan
Annotation
This article describes how the microbiome of a living host changes and transmigrates within the body after death, thus linking the microbiome of a living individual to post-mortem microbiome changes.
Abstract
Microbially mediated mechanisms of human decomposition begin immediately after death, and are a driving force for the conversion of a once living organism to a resource of energy and nutrients. Little is known about post-mortem microbiology in cadavers, particularly the community structure of microflora residing within the cadaver and the dynamics of these communities during decomposition. Recent work suggests these bacterial communities undergo taxa turnover and shifts in community composition throughout the post-mortem interval. Differences in the human post-mortem from the ante-mortem microbiome have demonstrated promise as evidence in death investigations. The current study investigated the post-mortem structure and function dynamics of Staphylococcus aureus and Clostridium perfringens after intranasal inoculation in the animal model Mus musculus L. (mouse) to identify how transmigration of bacterial species can potentially aid in post-mortem interval estimations. S. aureus was tracked using in vivo and in vitro imaging to determine colonization routes associated with different physiological events of host decomposition, while C. perfringens was tracked using culture-based techniques. Samples were collected at discrete time intervals associated with various physiological events and host decomposition beginning at 1 hour and ending at 60 days post-mortem. Results suggest that S. aureus reaches its highest concentration at 5–7 days post-mortem then begins to rapidly decrease and is undetectable by culture on day 30. The ability to track these organisms as they move into once considered sterile space may be useful for sampling during autopsy to aid in determining post-mortem interval range estimations, cause of death, and origins associated with the geographic location of human remains during death investigations. (Publisher abstract modified)
Date Created: December 26, 2017