Efficiency of this method was estimated by comparing number of copies in to copies out by quantitative polymerase chain reaction. PEC retention of DNA targets ranging 109©\288 base pairs (bps) in length was 15.88¨C2.14 percent (i.e., loss of 84.12¨C97.86 percent of target molecules). Experimental modifications of the PEC method resulted in no significant improvements; however, the benefit of PEC was revealed in its ability to remove most nontarget DNA molecules (99.99 percent). researchers also found that many (56.69 percent) of the target molecules were lost prior to their immobilization on the streptavidin -coated beads. These estimates of methodological efficiency are directly comparable to previous ones observed following fishing for DNA, an alternative method for DNA enrichment. (publisher abstract modified)
Downloads
Similar Publications
- Droplets Wicking in Thin Materials Exhibit Universal Drying Dynamics
- Evaluation of the Occurrence and Associative Value of NonIdentifiable Fingermarks on Unfired Ammunition in Handguns for Evidence Supporting Proof of Criminal Possession, Use and Intent
- The Role of Simulated Data in Making the Best Predictions (from the 87th Annual Meeting of the American Association of Physical Anthropologists - 2018)