The flexibility of the microfabricated format creates unique opportunities for study of the electrophoretic process. The present work utilizes digital images to capture the motion of DNA samples during pre-electrophoretic processes. A systematic study of DNA loading and strong sample stacking (sample concentration effects) was performed in order to analyze realistic DNA analysis conditions within microdevices. Using digital imaging and microscopy, DNA sample profiles within the injector were analyzed by deconvolving the geometrical intensity profile into different velocity groups. This analysis illustrates the evolution of molecular separation into distinct migrating populations within the injector itself. The present study performed DNA injections within microfabricated devices imposing run voltages between 85 and 850 V/cm. Data from 3 different offset lengths of a double-T cross-injector, 10 different applied voltages, and 2 different sample preparation protocols are presented.
(Publisher abstract provided.)
Downloads
Similar Publications
- Catching Career Criminals - A Study of the Repeat Offender Project - Technical Report
- Forensic Science and the Courts - The Uses and Effects of Scientific Evidence in Criminal Case Processing - Final Report
- Coping Patterns over Time and the Association with Stress, Depression and Self-Efficacy Among Adolescents: Latent Transition Analysis