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Concordance Study Between the AmpFlSTR MiniFiler PCR Amplification Kit and Conventional STR Typing Kits

NCJ Number
Journal of Forensic Sciences Volume: 52 Issue: 4 Dated: July 2007 Pages: 870-873
Date Published
July 2007
4 pages

This paper reports on a "concordance" (agreement or harmony) study that compared the DNA results for 1,308 samples evaluated with both the AmpFlSTR MiniFiler and Identifiler STR kits.


Full concordance between Identifiler and MiniFiler kits was found in 99.7 percent (10,437 out of 10,464) STR allele calls compared. The 27 differences found encompassed the loci D13S317 (n=14) and D16S539 (n=10) as well as D18S51 (n=1), D7S820 (n=1), and CSF1PO (n=1). The other three STR loci, D2S1338, FGA, and D21S11, and the sex determining locus amelogenin were fully concordant at all samples examined in this study. Three of the null alleles detected in the study were from children of fathers who also possessed the mutation that impacted the primer-binding site. Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed in 17 cases by reamplification of the samples and further testing using the PowerPlex 16 kit. DNA sequence analysis was also performed in order to determine the nature of the genetic variations that caused the allele dropout or apparent repeat unit shift. The 1,308 samples tested included 449 African-Americans, 445 Caucasians, 207 Hispanics, and 207 Asians. 1 table, 2 figures, and 9 references

Date Published: July 1, 2007