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Cathinone stability in authentic urine specimens

NCJ Number
253231
Date Published
May 2018
Length
7 pages
Author(s)
Lindsay Glicksberg; Sumandeep Rana; Sarah Kerrigan
Agencies
NIJ-Sponsored
Publication Type
Research (Applied/Empirical), Report (Grant Sponsored), Report (Annual/Periodic), Program/Project Description
Grant Number(s)
2013-R2-CX-K006
Annotation
Since earlier experimental studies using fortified urine have evaluated analyte, temperature, and pH-dependent variables associated with their stability, the current study compared experimental findings with those obtained using authentic urine from cathinone users.
Abstract
The study compared cathinone concentrations in 180 authentic unpreserved urine specimens, following known periods of refrigerated storage. These findings were compared with previously published experimental data, using fortified drug-free urine. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q/TOF-MS) was used to target 22 cathinones. Quantitative results were compared in urine specimens (pH 4.5-10) following 5-17 months of storage. The 180 specimens resulted in 164 quantitative findings involving a-PVP, ethylone, methylone, MDPV, and pentylone. Initial drug concentrations ranged from 25 ng/mL to over 100,000 ng/mL. Upon reanalysis, the percentage of drug remaining (0-119 percent) was correlated with storage time and specimen pH. The ability to reconfirm original results was not correlated with storage time. Instead, specimen pH was far more predictive. The relationship between initial and final drug concentration was highly pH-dependent, yielding significant correlations for a-PVP, ethylone and methylone, particularly under acidic conditions. These results agree with experimental findings and highlight the critical importance of specimen pH, rather than conventional time dependent variables, when considering cathinone stability in biological samples. The potential for pre-analytical changes in cathinone concentrations must be carefully considered when interpreting their results. (publisher abstract modified)
Date Created: July 20, 2021