DNA Analysis of LCN Samples: Towards Fully Integrated STR Profiling

The goal of the research is to adapt a rapid, fully integrated microfluidic sample-in to results-out system in development to allow Low Copy Number (LCN) DNA profiling. Specific objectives include: 1) developing a microfluidic post-PCR cleanup module, which will be optimized and integrated with the PCR amplification module to enhance signal strength from LCN samples, 2) improving the efficiency of Genebench's separation and detection component, 3) optimizing the PCR amplification module for higher sensitivity and for enhancing limit of detection, and 4) testing the optimized system components using mock evidentiary samples. The ultimate result of the will be a system that will generate a DNA profile in approximately 50 minutes from LCN sample introduction.

ca/ncf