This awardee has received supplemental funding. This award detail page includes information about both the original award and supplemental awards.
Description of original award (Fiscal Year 2004, $445,265)
The goal of this project is to study, design, and test new DNA sequencing methods using nanotechnology. The team at Brown University has extensive experience with synthesis of uniform carbon nanotubes (CNTs) and highly ordered CNT arrays. The increase in resolution that is expected to be seen with the nanotube arrays will promote the miniaturization of the device and will allow electrophoresis to be performed on a chip. The primary goals of this research are to increase the nanotube array throughput and to develop testing chips. The deliverable will be the development, optimization, and testing of carbon nanotube arrays for use in miniaturized, high-throughput DNA assays.
1. Examine a series of variables related to carbon nanotube synthesis, including the size of the nanotube array chip, the spacing of the nanotubes in the array, the surface properties of the nanotubes, the loading and injection of the DNA samples, the sensitivity, spacial resolution and speed of the read-out module, and the design of the microfluidic chamber.
2. Conduct a series of proof of concept studies of the first generation nanochips.
This is supplement #1 to award 2004-LT-BX-K001. Brown University will use this funding to study, design and test new DNA sequencing methods using nanotechnology. At the heart of DNA sequencing and the forensic sampling and analysis of DNA lies a true, tried, effective, but limiting method of DNA separation known as gel electrophoresis which Brown University hopes to displace through the proposed efforts. The size, time, costs, and cumbersome attributes of this technology warrant the investigation of a faster platform that can also work with smaller sample sizes. Typical gel electrophoresis separates DNA by hindering its movement in an electrical field by a polymeric matrix. Brown University will replace the polymeric matrix with the much smaller and much denser collection of carbon nanotubes (CNTs), thus allowing the ability to work with smaller samples of DNA that could be separated faster. In addition, the CNTs can be used repeatedly after washing and will speed up the sequencing process. The simplest to the most complicated cases could benefit from the routine and commonplace sequencing and comparison of DNA samples.
- Physics and Mathematical Models for Error Quantifications in Comparative 3D Microscopy for Physical Match Analysis
- A Universal Method for the Detection of Organic and Inorganic Gunshot Residue based on Fast Fluorescence Mapping and Raman Spectroscopic Identification
- The University of Alabama System and the Alabama Department of Forensics National Center on Forensics