The George Washington University (GWU) will create a public reference mitochondrial DNA (mtDNA) database for domestic dogs using mtDNA control region polymorphisms, as well as specific coding region polymorphisms that can resolve common haplotypes. A multiplex assay for the coding region SNPs will also be developed. Shed dog hair is often found at crime scenes, but contains little or no nuclear DNA to analyze. Presently, the extent of identification possible from hair found at a crime scene is by species type. GWU's project is designed to fully validate and entrench this forensic resource (shed canine hairs) as a tool in the arsenal of forensic analysis. The Principal Investigator was previously funded by the FBI to produce an initial canine mtDNA reference database.