Description of original award (Fiscal Year 2004, $644,251)
The goal of this DNA Research and Development project is to develop a breadboard microchip for DNA extraction, purification, and detection of single nucleotide polymorphisms (SNPs) using a primer extension assay (Taq-Man). Previous work by Cornell University has focused on the analysis of DNA from bacterial sources, and some of the components needed for this integrated system have been demonstrated for this purpose. Current methods for evaluating DNA evidence require time-consuming laboratory procedures that can significantly hinder the progress of active forensic investigation. The proposed system would rapidly extract and examine DNA from biological evidence in order to rapidly identify the source of the evidence and/or compare it to other samples. This technology is intended to augment the existing STR genotyping technology that is currently paired with the CODIS database. The portability of this system facilitates the acquisition of useful information from forensic evidence in the field in real time. This type of information could greatly accelerate the progress of an investigation.