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Developing a High-Yield DNA Isolation Method Using Matrix Proteinase for Compromised Skeletal Samples

Award Information

Award #
2003-IJ-CX-K105
Location
Awardee County
Walker
Congressional District
Status
Closed
Funding First Awarded
2003
Total funding (to date)
$25,001

Description of original award (Fiscal Year 2003, $25,001)

PROJECT SUMMARY 2003-IJ-CX-K105

This award will develope a modified to improve the yield of DNA isolation from bones samples by introducing a matrix proteinase treatment step after the grinding and prior to the actula DNA extraction. Yhe method is expected to increase the yield of DNA isolation and should lead to a higher success rate in generating
genotype profiles for the subsequent DNA analysis. The method will increase the ability of scientist and research to identify human remains of mass fatality incidents.

Date Created: September 29, 2003