Internal Validation of RapidHITID ACE Sample Cartridge and Assessment of the EXT Sample Cartridge*(dagger)

• NCJ Number: 254144
• Journal: Journal of Forensic Sciences Volume: 64 Issue: 3 Dated: 2019 Pages: 857-868
• Author(s): Gray D. Amick; Roy R. Swiger
• Date Published: 2019
• Length: 12 pages
• Grant Number(s): 2011-DN-BX-K564
• Agencies: NIJ-Sponsored
• Publication Type: Research (Applied/Empirical); Report (Study/Research); Report (Grant Sponsored); Program/Project Description
• Award: Forensic Science Training Development and Delivery Program

Annotation

Since a new rapid DNA solution, the RapidHIT ID, can accommodate two different sample cartridges, ACE, for the analysis of a single swab and EXT, for the analysis of DNA extracts, this article describes an efficient internal validation designed for low-throughput rapid DNA, along with an evaluation of the EXT sample cartridge.

Abstract

Each cartridge generated profiles with sufficient data quality to meet CODIS eligibility in fewer than 120 min. The results exhibited 100 percent correlation when compared to conventional DNA typing methods. Precision, reproducibility, stochastic, mixture, and contamination experiments produced expected results. Sensitivity of the ACE sample cartridge was acceptable for buccal swab analysis. The sensitivity of the EXT sample cartridge is discussed. The ACE validation and the EXT evaluation utilized a minimalist, cost-saving, efficient design to generate a validated RapidHIT ID instrument capable of producing genetic profiles from both extracted forensic DNA samples and buccal swab samples within 120 min. (publisher abstract modified)