Since there are several challenges for the extraction of DNA from the pollen grains, such as keeping the grain intact for secondary analysis and bypassing the issue of DNA mixtures more likely to be observed in a pollen assemblage, the current study developed a new method to address these challenges by extracting genetic material while not destroying the pollen grain on a single grain level.
The study demonstrated the method's efficiency with Pinus echinata, Taxodium distichum, and Plantago lanceolata single pollen grains. Quantitative PCR amplification of two genetic markers, ribulose bisphosphate carboxylase (rbcL) and internal transcribed spacer 2 (ITS2), and digital microscopy demonstrated the non-destructive nature of this novel DNA extraction method from single pollen grains. (publisher abstract modified)
Downloads
Similar Publications
- Restorative Justice and School-wide Transformation: Identifying Drivers of Implementation and System Change
- Coping Patterns over Time and the Association with Stress, Depression and Self-Efficacy Among Adolescents: Latent Transition Analysis
- Just Science Podcast: Just Mass Disaster Emergency Response in Maui, Hawaii