Description of original award (Fiscal Year 2009, $241,117)
This project will develop devices and methods for single-cell forensic short tandem repeat (STR) typing using a microfabricated single-cell genetic analyzer (SCGA) that integrates single-cell capture, nanoliter-volume PCR amplification, integrated quantitative post-PCR sample cleanup, inline injection and capillary electrophoresis (CE) separation. Further evaluation of this microsystem for forensic mixture and low-copy-number DNA analysis will be performed by typing non-probative samples. Finally, this new method will be critically tested and disseminated with the help of operational crime laboratories.
- Improving identification of unknown American Indians and Hispanic/Latinx Americans
- Predictive tools and experiments for blood backspatter: incorporating tissue simulants and muzzle gas interactions
- Data fusion from infrared elemental, MSP and Raman analysis techniques to the maximization of the efficiency of the analytical sequence for the forensic examination of paint evidence