SpermPaint Optimization and Validation

The microscopic identification of sperm in sexual assault evidence can be labor intensive and time consuming. During the initial phase of this project, fluorescently labeled anti-sperm antibodies were developed. These antibodies (called "Sperm Paints") have the capability to bind to sperm and make them easily visible using a fluorescent microscope. The ability of the Sperm Paints to bind to and illuminate sperm heads and tails in simulated sexual assault evidence that has been stored for prolonged periods has also been demonstrated. The goal of this supplemental funding is to complete the development and optimization of a protocol for using the Sperm Paints for microscopic sperm searches, and to perform testing and validation of the Sperm Paints through collaborative studies with crime labs.

Objectives:

1. Optimize sperm binding by a cocktail of fluorescent monoclonal anti-sperm antibodies (Sperm Paint) which bind to sperm specific proteins located in different domains of the sperm head and tail.
2. Determine the binding characteristics of Sperm Paint to simulated evidence obtained 24, 48, 72 and 96 hours post-coitus.
3. Collaborate with crime labs to evaluate and validate the Sperm Paints.
4. Develop an optimized protocol for Sperm Paint use.

ca/ncf