This webinar discusses the essential aspects of best practice using liquid chromatography diode array detector (LC-DAD) with optional electrospray ionization mass spectrometry (ESI/MS) for compliance testing of hemp and hemp-derived products. The key topics of this webinar will include: 1) an overview of the most effective LC separation published so far; 2) a description of a simple methanol extraction procedure, consisting of homogenization, ultrasonication/pulverization, centrifugation, filtration, and dilution, to recover cannabinoids from a wide range of samples; 3) how to utilize ultraviolet (UV) detection at multiple wavelengths for optimal sensitivity and improved selectivity; and 4) how to confirm identity of cannabinoids by optional ESI/MS using either high or low-resolution mass spectrometry.

This webinar was hosted by the NIJ Forensic Technology Center of Excellence on June 13, 2024.