A Low Cost Microfluidic Instrument for Typing SNPs

The goal of this work is to design, build, and test an integrated microfluidic controlled microarray test for single nucleotide polymorphism (SNP) typing. Akonni previously demonstrated the feasibility of a gel drop microarray platform as a complete solution for forensic SNP-typing applications. Sample preparation, polymerase chain reaction (PCR), and the microarray were packaged into prototype flow-through microfluidic modules for Y-chromosome SNP determination. The current work will focus on: 1) refining protocols and assay chemistries and 2) packaging components and reagents into an integrated system for automated, sample-to-answer results.

ca/ncf