Prior to setting up an amplification reaction, the analyst must ensure that reaction tubes are compatible with the thermal cycler used. Each thermal cycler manufacturer can provide information regarding the size and type of reaction tubes that are recommended. Sample reaction volumes generally range from 25-100µL, although many forensic laboratories have successfully validated reactions with less than 25µL. The thermal cycler used may limit the choices of reaction tubes and sample reaction volumes (e.g., the 480 thermal cycler requires PCR reaction tubes with no lid to allow a mineral oil layer).

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