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DNA Amplification for Forensic Analysts

Factors

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Photo of test tubes
National Institute of Justice (ver política de reutilización).

Designing successful multiplexes can be challenging. The process begins by deciding which STR loci are suitable to incorporate into a multiplex.

Factors in deciding the loci include:

  • Locus size and overlap
  • Number of alleles
  • Thermal cycling parameters
  • Primers
  • Dyes

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