GeneScan^® is a sophisticated software program that converts raw data to analyzed data through the application of a size standard, a matrix file, and specific parameter settings.

GeneScan^® software sizes peaks using the internal size standard added to the sample prior to separation, as shown below.

While samples are being processed by the genetic analyzer, sample files (.fsa) are generated during the data collection process. These files are then analyzed by the GeneScan^® analysis software. There are five vital pieces of information in the sample file that are used and displayed during GeneScan^® data analysis: (select the names below to see an example screen.)

• Ept file
• Raw data
• Size calling curve
• Sample file information
• Analyzed data

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