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DNA Extraction and Quantitation for Forensic Analysts

Modifications

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Many extraction procedures incorporate the use of a microcentrifuge tube with a spin basket.  Costar Spin-X® centrifuge tube filters are one example.  These centrifuge tube inserts contain no membrane, but have a bonded fritted bottom on the 500µL insert that fits into the microcentrifuge tube.1,2

This allows for the separation of liquid carrying the biological material from the substrate during centrifugation steps.  Another modification is the use of centrifugal filter units.  These allow for extracted DNA to be purified and concentrated after extraction and prior to amplification. Millipore Microcons® are one example of centrifugal filter units.2 Both of these modifications are routinely used in DNA extraction procedures.

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