DNA extraction

What’s Possible with Rapid DNA Technology?

August 2022

Series

NIJ scientist Tracey Johnson joins science writer Sarah Michaud in this episode. They discuss Rapid DNA technology, and Tracey explains the complexities of this technology – its pitfalls and its possibilities.

Reading and Resources from NIJ:

Alternative direct-to-amplification sperm cell lysis techniques for sexual assault sample processing

Date Published

May 2022

Publication Type

Research (Applied/Empirical)

Agencies

NIJ-Sponsored

Micromanipulation of single cells and fingerprints for forensic identification

Date Published

2021

Agencies

NIJ-Sponsored

The effects of dithiothreitol (DTT) on fluorescent qPCR dyes

Date Published

2021

Agencies

NIJ-Sponsored

Rapid Genotyping Device for Complex DNA Samples

Date Published

June 2013

Publication Type

Research (Applied/Empirical), Report (Study/Research)

Agencies

NIJ

The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs

Date Published

2015

Agencies

NIJ-Sponsored

Evaluation of automatable silica-based extraction methods for low quantity samples

Date Published

February 2022

Agencies

NIJ-Sponsored

Integrated Sample Cleanup-Capillary Electrophoresis Microchip for High-Performance Short Tandem Repeat Genetic Analysis

Date Published

2008

Agencies

NIJ-Sponsored

Development of a quantitative PCR-based method for studying temporal DNA degradation in waterlogged bone

Date Published

2020

Agencies

NIJ-Sponsored

Rapid DNA Crime Scene Sample Multi-Laboratory Evaluation Project

DJO-NIJ-21-RO-0004

Open

Funding First Awarded

2021

$475,000

Assessing Heteroplasmic Variant Drift in the mtDNA Control Region of Human Hairs Using an MPS Approach

Date Published

January 2018

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

Assessment of Impact of DNA Extraction Methods on Analysis of Human Remain Samples on Massively Parallel Sequencing Success

Date Published

January 2019

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

Sperm Cell Purification From Mock Forensic Swabs Using SOMAmer (TM) Affinity Reagents

Date Published

July 2018

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

Targeted Multiplexed Next Generation RNA Sequencing Assay for Tissue Source Determination of Forensic Samples

Date Published

December 2015

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

Pressure-Based Alkaline Lysis With Immunocapture, a Method for Enhanced Recovery in Differential Extraction

Date Published

2017

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

A Capillary Electrophoresis Method for Identifying Forensically Relevant Body Fluids Using miRNAs

Date Published

January 2018

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

In Vitro Isolation of Small-Molecule-Binding Aptamers With Intrinsic Dye-Displacement Functionality

Date Published

2018

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

NOCIt: A Computational Method To Infer the Number of Contributors to DNA Samples Analyzed by STR Genotyping

Date Published

November 2014

Publication Type

Research (Applied/Empirical)

Agencies

NIJ-Sponsored

Forgotten Evidence: A Mixed Methods Study of Why Sexual Assault Kits (SAKs) Are Not Submitted for DNA Forensic Testing

Date Published

October 2017

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

A Broadly Applicable Assay for Rapidly and Accurately Quantifying DNA Surface Coverage on Diverse Particles

Date Published

2017

Agencies

NIJ-Sponsored

Inter-Sample Contamination Detection Using Mixture Deconvolution Comparison

Date Published

May 2019

Publication Type

Research (Applied/Empirical), Report (Study/Research), Report (Grant Sponsored), Program/Project Description

Agencies

NIJ-Sponsored

A Rapid, Microfluidic Transcriptomic Method for Body Fluid Identification from Forensic Samples

2020-DQ-BX-0027

Open

Funding First Awarded

2021

$436,934

An Affinity-Free, Centrifugal Microfluidic System for Rapid, Automated Differential Extraction

2020-DQ-BX-0024

Open

Funding First Awarded

2021

$466,886

Selectively analyzing and interpreting DNA from multiple donors with a full Single-Cell strategy

2020-R2-CX-0032

Open

Funding First Awarded

2020

$142,986

Modelling Heterozygote Balance in Forensic DNA Profiles

Date Published

December 2012

Publication Type

Report (Study/Research)

Agencies

NIJ-Sponsored

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