Desomorphine, a semi-synthetic opioid, is a component of the street drug Krokodil. Despite continued reports of Krokodil use, confirmation via toxicological testing remains scarce. In the current project, Solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS) were used to determine desomorphine in blood and urine, using a deuterated analog as the internal standard. Data were acquired using selected ion monitoring (SIM) mode. Extraction efficiencies in blood and urine were 69 percent and 90 percent, respectively. The limits of quantitation in blood and urine were 5 ng/mL and 8 ng/mL, ten-fold lower than previously published methods. Intra- and inter-assay CVs were 2-4 percent (n=3) and 3-7 percent (n 15), respectively. The method was fully validated in accordance with published guidelines for forensic use, and it provides a means for identifying desomorphine in toxicology specimens at forensically relevant concentrations, without the need for derivatization. (publisher abstract modified)
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